Fluorescent Cell Counting

Bright field green fluorescent and red fluorescent channels were used.

Fluorescent cell counting.

The amount of a fluorescent dye calcein produced from calcein am 3 6 di o acetyl 2 7 bis n n bis carboxymethyl aminomethyl fluorescein tetraacetoxymethyl ester by esterases in cells is directly proportional to the number of. If you have a cell counter with fluorescent capabilities like we do there is a way around it without adding additional steps to the protocol. Because the concentration of beads is known the volume of sample analyzed can be easily computed. The sample is run through a flow cytometer and set to stop after a predetermined number of beads are analyzed.

Add an appropriate amount of trypsin. Wash your cells with pbs. A rectangular section near the analyzed cell that is the non fluorescent part of the image is selected which can be used to minimize the maximum errors in the fluorescent counting of a cell. Here is our protocol for cell counting with a twist.

Cell counting using a fluorescent dye. Fluorescent cell counting measure rfp positive cells in samples containing gfp rfp and non expressing cells live cell analysis of a 96 well plate containing samples with varied of rfp gfp and non expressing hela cells were counted using celigo. Fluorescent cell counting kit fcck is utilized for the fluorometric detection of living cell numbers.